Device
Part:BBa_K1627001:Design
Designed by: Alejandro Gutierrez Group: iGEM15_Austin_UTexas (2015-09-10)
Device to demethylate 3-methylxanthine
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 644
Illegal NheI site found at 839 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1207
Illegal BglII site found at 1678
Illegal XhoI site found at 2819 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3517
Illegal AgeI site found at 1346 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
These genes were amplified from the pDCAF3 plasmid (BBa_K734000). An EcoRI restriction site was removed from the ndmB gene during the cloning process. The genes were cloned into the pSB1C3 backbone using BioBrick Standard Assembly.
Source
The ndmB and ndmD genes were isolated from Pseudomonas putida CBB5. gst9 was isolated from Janthinobacterium sp. Marseille.